Characterization of the glutathione S-transferase genes in the sand flies Phlebotomus papatasi and Lutzomyia longipalpis shows expansion of the novel glutathione S-transferase xi (X) class

Ashraf, F and Weedall, GD (2022) Characterization of the glutathione S-transferase genes in the sand flies Phlebotomus papatasi and Lutzomyia longipalpis shows expansion of the novel glutathione S-transferase xi (X) class. Insect Molecular Biology. ISSN 0962-1075

Preview

Text Characterization of the glutathione S‐transferase genes in the sand flies.pdf - Published Version Available under License Creative Commons Attribution. Download (6MB) | Preview

Abstract

Leishmaniasis control often relies upon insecticidal control of phlebotomine sandfly vector populations. Such methods are vulnerable to the evolution of insecticide resistance via a range of molecular mechanisms. There is evidence that two major resistance mechanisms, target site insensitivity and metabolic resistance, have evolved in some sandfly populations and further genetic characterization of resistance would be useful to understand and combat it. To facilitate the study of the mechanisms of metabolic resistance, here we improved the annotation and characterized a major detoxification gene family, the glutathione-s-transferases (GST), in the genomes of two sand fly species: Phlebotomus papatasi and Lutzomyia longipalpis. The compositions of the GST gene family differ markedly from those of Aedes and Anopheles mosquitoes. Most strikingly, the xi (X) class of GSTs appears to have expanded in both sand fly genomes. Our results provide a basis for further studies of metabolic resistance mechanisms in these important disease vector species.

Item Type:	Article
Uncontrolled Keywords:	Science & Technology; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; Entomology; detoxification; insecticide resistance; Leishmaniasis; Lutzomyia longipalpis; metabolic resistance; Phlebotomus papatasi; sand flies; INSECTICIDE SUSCEPTIBILITY STATUS; ORIENTAL FRUIT-FLY; BIOINFORMATICS RESOURCE; ANOPHELES-GAMBIAE; ENDEMIC AREAS; RESISTANCE; EXPRESSION; VECTOR; IDENTIFICATION; DDT; Leishmaniasis; Lutzomyia longipalpis; Phlebotomus papatasi; detoxification; insecticide resistance; metabolic resistance; sand flies; 06 Biological Sciences; Entomology
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Divisions:	Biological and Environmental Sciences (from Sep 19)
Publisher:	Wiley
Date of acceptance:	21 February 2022
Date of first compliant Open Access:	29 April 2022
Date Deposited:	29 Apr 2022 09:10
Last Modified:	29 Apr 2022 09:15
DOI or ID number:	10.1111/imb.12769
URI:	https://ljmu-9.eprints-hosting.org/id/eprint/16731

View Item