Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Krzemień, P, Kasperczyk, S, Banach, M, Kasperczyk, A, Dobrakowski, M, Tomasik, T, Windak, A, Mastej, M, Catapano, A, Ray, KK, Mikhailidis, DP, Toth, PP, Howard, G, Lip, GYH, Tomaszewski, M, Charchar, FJ, Sattar, N, Williams, B, MacDonald, TM, Penson, PE et al (2022) Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress. Biomarker Insights, 17. ISSN 1177-2719

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Abstract

Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress.
Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing.
Results: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P = .038) and 11% lower concentration of UA (P = .005). TOS was 20% lower (P = .014). The activity of SOD was up to 5% higher (P = .037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity.
Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes.

Item Type:	Article
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Sage
Date of acceptance:	19 November 2021
Date of first compliant Open Access:	1 February 2022
Date Deposited:	01 Feb 2022 12:43
Last Modified:	01 Feb 2022 12:45
DOI or ID number:	10.1177/11772719211066791
URI:	https://ljmu-9.eprints-hosting.org/id/eprint/16207

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